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Phylogenetic analysis of the oxygenases in the alp , flu and lom gene clusters demonstrates that the enzymes predicted to be involved in the A-ring transformation (Alp1S and Alp1V, Flu21 and Flu29, and Lom16 and Lom17, respectively) with the exception of Flu29 are clustered in a branch distinct from those well-characterized oxygenases involved in biosynthesis of angucycline antibiotics, suggesting that these oxygenases (represented by Alp1S and Alp1V) catalyse similar A-ring transformation reactions, that is, a C4-hydroxylation and a C2, C3-epoxidation, and that Flu29 is probably the epoxidase working on an isomerized fluostatin intermediate (Supplementary Fig

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