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3,000 SH-SY5Y MKK7 ACT cells were plated in 96-well plates in DMEM with 10% FBS

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However, Pib2 (phosphatidylinositol 3-phosphate-binding protein 2), the proposed glutamine sensor in yeast (Ukai et al., 2018), is not conserved in humans, and the closest candidates Phafin1 or R3HCC1 (R3H and coiled coil domain–containing protein 1) did not affect glutamine signaling to mTORC1 (Meng et al., 2020)