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This pH shift could suggest that the initial pH drop is attributed to the rapid consumption of pyridine by the reaction with Ac 2 O, resulting in the formation of acetic acid and acetylpyridinium, which could react gradually with the α-carboxy group of isoAsp, forming a mixed anhydride IV and regenerating pyridine, causing the gradual pH rise

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Association between ambient temperature and semen quality: A longitudinal study of 10–802 men in China