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Briefly, 10 μ L of each sample (containing 2 mg/mL total protein) was loaded onto 5% staking and 8% native polyacrylamide gel bathed in 1 × Tris-glycine buffer (pH 8.3) and the proteins separated at constant current (120 V) at 4°C for 3 hours

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It's associated with a range of health risks, including neurodegeneration, mitochondrial dysfunction, impaired detoxification, toxin accumulation, malignancy, and increased antioxidant need