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Each sample, in duplicate, was amplified as follows: one cycle of 95°C for 1 minute for pre-incubation, followed by 40 cycles of 95°C for 30 seconds, 60°C for 1 min and 72°C for 1 minute, with a subsequent melting curve analysis using Mx3000p (Stratagene, Agilent Technologies, CA, USA)

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