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Cells were grown at 37 °C to an optical density at 600 nm (OD 600 ) of 0.7–0.8 in lysogeny broth (LPS solution LB-05) medium, and then 0.5 mM of isopropyl-1-thio-β-D-galactopyranoside (LPS solution IPTG025) was added

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If you have any of the following medical illnesses or conditions, you must tell your doctor and your registered nurse