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Although the pathophysiology of migraine is still not completely clear, in recent years there have been important advances in knowledge about mechanisms that help explain it and new therapeutic targets that have led to the availability of new pharmacological treatments [7]

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The cartridges were washed with 50 μl of 80% acetonitrile/0.1% TFA, and the cross-linked peptides were eluted with 25 μl of a 3:1 ratio of water:NH 4 OH into a 96-well LoBind plate containing 25 μl of 10% formic acid in water to immediately neutralize the pH