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Deionized water was freshly prepared using a Thermo Scientific system and was used for reagent formulations, reaction mixtures, and AgNP synthesis

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A clear demonstration was obtained that BPC 157 stimulated both the egr-1 gene (critical in proliferation, differentiation, and inflammation during cholestatic liver injury, cytokine and growth factor generation, and early extracellular matrix (collagen) formation) (Kim et al., 2006