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The soluble fraction was clarified by centrifugation (75,000 × g , 20 min, 4 °C) and incubated with 50% Ni-Sepharose suspension (GE Healthcare, Chalfont St Giles, UK) for 2 h at 4 °C with gentle rotation prior to affinity purification

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Electrolyte Flavour:Citrus Twist

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