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The procedure itself is simple: you relax in a private room while the IV drip is administered by a registered nurse over 30-60 minutes

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Cell culture and analysis JEG3 human choriocarcinoma cells JEG3 cells (ATCCHTB36 TM ) were cultured in six-well plates or coverslips in 20% O 2 at 37°C in Eagle's Minimum Essential Medium (EMEM) media (ATCC, 30-2003) containing FBS and penicillin–streptomycin (Visent Inc)