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Fractions containing 26S proteasomes were pooled and concentrated to ~50–100 µl before freezing as 10-µl single-use aliquots in liquid N 2 and stored at −80 °C

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This synthesis of evidence will not only offer valuable insights for researchers and clinicians but also identify gaps in knowledge and suggest directions for future investigation, ultimately aiming to advance the translation of gut-brain axis research into effective clinical applications for neurological diseases