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For stomach lavage, stomach, small intestine, colon and small intestine lavage (calibration samples and QCs (of the respective matrix) or PK samples), 50 µl of a sample was extracted with 50 µl of a 50:50 mixture of acetonitrile and methanol containing 12.5 ng ml −1 of caffeine as internal standard for 15 min at 800 rpm on an Eppendorf MixMate vortex mixer