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Cells were washed three times with PBS and subsequently resuspended in 5 ml of buffer A, which contained 10 mM HEPES (pH = 7.9), 1.5 mM MgCl2, 10 mM NaCl, 0.5 mM DTT, and protease inhibitor cocktail (Sigma-Aldrich, GE80-6501-23)

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We can divide the different forms in which vitamin B12 is found into two groups: Other forms: aquocobalamin, nitritocobalamin