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Both technologies can be used for analyzing short strands of bisulfite-treated DNA just as the old techniques, but the main advantage is that bisulfite treatment is no longer required for sequencing DNA, as ONT and SMRT can detect many types of DNA modifications simultaneously on long strands, allowing genome, epigenome, transcriptome and epitranscriptome profiling without specific prior sample preparation [25–27]

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Clin Biochem 2014