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Pellets were resuspended in DMEM/F-12 medium (Life Technologies, Carlsbad, CA, USA) supplemented with 20% foetal bovine serum (FBS, Pan Biotech, Aidenbach, Germany) and penicillin/streptomycin (Life Technologies) and plated on 10 cm cell-culture dishes

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Recently, it has been demonstrated that glutaminolysis, which also supplies substrates for the Krebs cycle, facilitates the activation of OXPHOS and ATP production, thereby regulating efferocytosis efficiently through a non-canonical glutamine metabolism