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Non-parametric identification of spatially associated pathway To relax the assumption of a 2 nd order spatial regulation function, we estimated pathway expression by summing up, in each cell, the UMIs of genes from pathways (WikiPathways, Reactome) and known complexes (Corum) was summed up and normalized to the total number of UMIs in each cell to estimate the fractional abundance of RNAs from that complex/pathway within each cell’s transcriptome

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