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In a suitable tube (50 ml) layer 10 ml of each of the two gradient solutions and underlayer these with 10-15 ml of the sample-OptiPrep™ mixture (density approximately 1.17 g/ml), to form a three-step gradient (see Figure 1 and Notes 4 and 5)

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Additionally, a significant improvement in liver function and reduced oxygen requirement was also observed in all ten patients enrolled in the Ibrahim et al