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Previous research has demonstrated that AQP4-IgG can induce the release of various proinflammatory mediators, including IL-1β, IL-6, and TNF-α, by both astrocytes and microglia 25,26,27 .To evaluate the potential of IGF-2 treatment in reducing neuro-inflammation, we conducted an ELISA assay to measure the expression levels of IL-1β, IL-6, and TNF-α in the serum of mice subjected to different experimental conditions