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Extracellular vesicle separation methods have been developed based on their biophysical properties (size, density, charge, and composition), including ultracentrifugation, ultrafiltration, size exclusion chromatography, polymer precipitation, and immunoaffinity approaches, among which ultracentrifugation represents the gold standard for extracellular vesicle isolation and is well established and widely adopted by the research community

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Proteomics of synechocystis sp