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the guide cannula measured 2.5 cm in overall length and was prepared with a heat bulge placed 2.5 mm from its beveled tip, thus acting as a stop to control the depth of penetration at 2.5mm, Once in position, the cannula was secured to the skull with two stainless-steel screws and dental cement

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Swiss, C57BL/6, BALB/cJ, ICI, CD1, SJL mice) at different dietary doses ranging from 0.1% to 5% revealed a highly species- and strain-dependent susceptibility to CPZ-induced demyelination, and the extent of demyelination also varied with dose, age, sex and brain region (Skripuletz et al., 2011