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Nuclear and cytoplasmic protein of TM3 cells was extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions

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Proteasomal dysfunction is believed to play a role in the pathogenesis of PD because ubiquitin builds up in LBs and interacts with the proteasomal system through interactions with parkin and UCH-L1