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For Masson staining analysis, the collagen fibers’ staining area and the total area of each field (magnification: 10×) were analyzed using Image J software (National Institutes of Health, Bethesda, MD, USA), then we calculated the collagen volume fraction (collagen volume fraction = area of collagen fibers’ staining/total area of the field × 100%)

have not been evaluated by the FDA and are not intended to diagnose