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Deletion of egtA and yap1 and complementation of the ∆ egtA and ∆ egtA ∆ yap1 strains For generating egtA and yap1 deletions in AfS77, and the associated double mutants, the bipartite marker technique was used 61

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Collectively, our results identified that the MMSET gene was crucial for promoting MM ferroptosis sensitivity and demonstrated the important role of PUFAs in regulating MMSET-induced sensitivity to ferroptosis