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Researchers isolated the parent protein from gastric juice and identified this fragment as the biologically active portion in animal studies

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(2001) — Proceedings of the National Academy of Sciences (PNAS) , 98(22): 12438–12443 — Foundational study demonstrating that peptides from the amino-terminal MDM-2 binding domain of p53, designed from conformational analysis, are selectively cytotoxic to transformed cells, establishing the basis for PNC-27’s design