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The next day, 10 ml of medium was inoculated with the overnight culture, cells were grown until an OD 600nm = 0.6 and protein expression was induced with 1 mM isopropyl-β-D-thiogalactoside, upon which the cells were grown for 4 h at 20°C

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Lipoic acid increases de novo synthesis of cellular glutathione by improving cystine utilization