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–34.9 kcal/mol), and the ΔG°′ required for ATP formation from ADP and P i : 2 × 30.5 kJ/mol = 61 kJ/mol (2 × 7.3 kcal/mol = 14.6 kcal/mol)

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Sirtuins, which are NAD+-dependent deacetylases, affect many biological processes including inflammation, circadian rhythms, and cellular stress responses2