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An equivalent amount of protein from each animal was mixed with 4 × sample buffer with or without 2-mercaptoethanol, then separated by SDS–polyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

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En un estudio notable, ratones hembra envejecidos que recibieron Epithalon mostraron un aumento del 12.3% en la esperanza de vida media comparados con los grupos control