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The following mechanisms of free-radical scavenging may be proposed for the α-lipoic acid/dihydrolipic acid redox couple: Hydrogen atom transfer mechanism: Hydrogen peroxide Proton loss electron transfer mechanism: Single electron transfer followed by proton transfer mechanism: Lipoic Acid Metabolism Lipoic acid is an essential cofactor for mitochondrial metabolism and is synthesized de novo using intermediates from mitochondrial fatty acid synthesis type II, S -adenosylmethionine, and iron–sulfur clusters

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Temperature proves most critical - every 10°C increase doubles degradation rate, meaning room temperature storage (25°C) degrades peptides 4-8x faster than refrigeration (2-8°C) and dramatically faster than freezing (-20°C or below)