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A very illustrative example is the increased bacterial translocation (BT) observed in numerous pathologies and that is defined as the migration of viable micro-organisms and bacterial components from the gut lumen toward the mesenteric lymph nodes (MLNs) and extra-intestinal sites such as the peritoneal cavity, liver, spleen and kidney ( The Paracellular Route Evidences for the role of TJs as key regulators in the epithelial barrier are based historically on observation from two very different fields: electron microscopy (EM) and electrophysiology