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A more specific guideline for iEC molecular and functional validation will be necessary for using these valuable models studying the detrimental factors to EC dysfunction under disease conditions and subsequent drug screening

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Moreover, clinical trials have confirmed that unlike the MMR vaccine, which relies on γδ T cells to induce TI, the protective effect of BCG is primarily mediated by epigenetic reprogramming of macrophages, highlighting the cell-type specificity of TI induction across different vaccines [41, 42]