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For growth factor stimulation, serum-deprived cells were cultured as needed, with 0.9 µM insulin (Sigma-Aldrich, Louis, MO, USA) or 12.9 nM FGF (200 ng/mL, #F5542, Sigma) and 50 ng/mL heparin (#9041-08-1, Aladdin), for 15 min

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It’s also a relatively quick process