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4 After pre-incubation in a shaker at 37 °C for 5 min, a NADPH-generating system (NADP 6.5 mM, glucose 6-phosphate 16.5 mM, MgCl 2 16.5 mM, and glucose 6-phosphate dehydrogenase 2 U/mL) was added to initiate the reaction

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