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Moreover, compounds associated with nucleotide metabolism, such as 5-methylthioadenosine, xanthine, and hypoxanthine, were also elevated, alongside phenylalanine metabolism intermediates like phenylacetaldehyde and folate metabolism-related substances including 4-aminobenzoic acid

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A protease cleavage site can also be included as part of this linker to allow for removal of the His-tag