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Lysates were sonicated 10 times with 1-s pulses at 80% amplitude on a Fisher Scientific Model 120 Sonic Dismembrator (Thermo Fisher Scientific), then centrifuged at 16,000 g at room temperature (for 9 M urea) or at 4°C (for RIPA lysis) for 15–20 min

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It was found that the NPs promoted CCL5 degradation through NF-κB signaling and induced the conversion of immune dendritic cells (igDCs) in the spleen to tolerogenic dendritic cells (tDCs)