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VSMC phenotype was similarly preserved in Imatinib-treated Tβ4 –/Y aortas, with increased expression of contractile markers Calponin 1 (Western blot, Supplemental Figure 13C), αSMA (immunofluorescence, Figure 11C), and Sm22a (qRT-PCR, Figure 11D) and a corresponding decrease in expression of synthetic markers, Caldesmon (immunofluorescence, Figure 11C) and Tropomyosin (qRT-PCR, Figure 11D)

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Finally, we simulated a final score for each ASM participant using the formula proposed in section III.C.2.e