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Briefly, adult fat bodies were dissected in 1× phosphate buffered saline (PBS), fixed 20 min in 4% paraformaldehyde at room temperature (RT), rinsed 5× in 1× PBS, then permeabilized for 30 min at RT in 1xPBS + 0.3% Triton X-100

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FDX1 further interacts with lipoyl synthase (LIAS) to facilitate the lipoylation of DLAT [67]