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For immunoprecipitation (IP), 293FT cells were transfected with indicated plasmids by lipo293, after 48 h, cells were harvested and lysed in lysis buffer A (10 mM HEPES pH7.5, 1.5 mM MgCl2, 10 mM KCl,0.5 mM DTT, 10 mM protease inhibitor cocktail, 10 mM phosphatase A inhibitor, 1 mM PMSF)