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In agreement with the proposed role of THUMP domains in the recognition of the 3′-CCA tail of tRNA substrates, the human THUMPD3-TRMT112 complex cannot introduce m 2 G into a tRNA substrate lacking the 3′-CCA tail (Yang et al., 2021)

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Without adding CEB-B solution to produce the Nucleus2 and Cytoplasm2 fraction without nuclear membrane proteins