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Instead of shedding normally, dead skin cells stick together and accumulate within pores, contributing to blockages that lead to blackheads and whiteheads

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Quantitative Real-Time PCR (qRT-PCR) was used to measure mRNA expression of SASP factors (IL-1β, IL-6, TNF-α, TGF-β) in TM3 cells, normalizing data to GAPDH