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[DOI] [PubMed] [Google Scholar] 28.Young AA, Vine W, Gedulin BR, Pittner R, Janes S, Gaeta LSL, Percy A, Moore CX, Koda JE, Rink TJ, Beaumont K

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Since INS-1E cells are rat-derived, we used the mRNA sequence encoding rat α 2A -adrenergic receptor as a template to generate guide RNA (gRNA) to disrupt the promoter region of the Adra2a gene (gRNA sequence: 5′-GCAGCCGGATGCCGGCAATA-3′, positions 57–76)