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2,5-Dioxopyrrolidin-1-yl-3-(prop-2-ynyloxy)propanoate (NHS-ester-alkyne) was obtained from Sigma-Aldrich

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The GPX4 activity depends on GSH, synthesized from cysteine imported through the Xc − (SLC7A11/SLC3A2) transporter ( − deplete cysteine and GSH, leading to GPX4 inactivation and accumulation of lipid peroxide, and trigger ferroptosis