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E2 appears to exert anorexigenic effects, whereas P4 in the presence of E2 tends to increase appetite and preference for energy-dense foods, an effect further modulated by changes in leptin, ghrelin, peptide YY (PYY), and possibly central insulin signaling [114]

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DNA concentration was quantified using a Qubit 3.0 fluorometer, and the purified DNA was subsequently used for qPCR