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Apply sufficient pressure on the tube to discharge a thin line of ointment roughly half an inch (1 centimetre), or as directed by your doctor, pharmacist, or optician

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FVS 700 (live/death dye, #564997, BD Bioscience, CA, USA), CD3e (#562600, BD Bioscience), CD8a (#557654, BD Bioscience), F4/80 (#565411, BD Bioscience), CD86 (#558703, BD Bioscience), CD163 (#12-1631-82, Thermo Fisher), FAP (#FAB9727G, R&D system) and Pdgfra (#12-1401-81, Thermo Fisher) were used in experiments