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Experimental methods for evaluating these markers including immunohistochemistry staining for DNA damage markers histone variant γ H2A histone family member X (γH2AX), P53-binding protein 1 (53BP1), and promyelocytic leukemia (PML), the loss of nuclear protein lamin B1, intracellular absence of high mobility group box 1 (HMGB1) and bulk RNA-seq profiling for SASP factors

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Treatment with intramuscular injection of vitamin B12 was initiated (1000 mcg IM twice a week for a month, plus 100 mcg orally per day)