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Construction of recombinant viruses Recombinant viruses Venus-iUL49 or MEF-iUL49 expressing iUL49 fused to a Venus or MEF (for Myc tag, the Tobacco Etch Virus protease cleavage site, and Flag) tag 52 , Venus-iUL44b and SE-vdUTPase expressing UL50 (vdUTPase) fused to a Strep-tag (Supplementary Figs