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The rationale for combining these peptides extends beyond simple additive effects, as BPC-157's gastric pentadecapeptide sequence activates growth factor pathways and nitric oxide systems while TB-500's thymosin beta-4 derivative promotes actin regulation, cell migration, and blood vessel formation through separate receptor interactions

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The animals were then deeply anesthetized with 3% isoflurane and 50 mg/kg of ketamine followed by a laminectomy at lumbar level L2-L3, corresponding to the sacrocaudal spinal cord