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The NCLX transporter carries out electrogenic transport of four Na + ions into the mitochondria in exchange for one Ca 2+ ion and one K + ion out of the mitochondria

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Cells were cultured in the RPMI-1640 medium supplemented with 10% fetal calf serum, 10 mM HEPES, 2 mM L-glutamine, 1 mM sodium pyruvate, 0.05 β-ME and 5.0 µg/mL puromycin, and grown at 37 °C with 5% CO 2 , as described 62