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Cytotoxicity in mammalian cells Vero cells (African Green Monkey Kidney cells, ATCC CCL-81) were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing glucose, L-glutamine and sodium pyruvate and supplemented with 10% fetal bovine serum and 1X penicillin–streptomycin (Sigma) at 37 °C in culture flasks (TPT-90025) until confluent growth was attained
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Extracellular dipeptidase then degrades the Cys-Gly dipeptide and releases Cys as well as Gly
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