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Glossitis and hyperpigmentation of hands due to Vitamin B12 deficiency. ⬇️ B12 leads to ⬆️ melanin synthesis more frequently in people with darker skin. Skin hyperpigmentation was reversed with B12 replacement

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However, these spacers should be long enough to ensure that the tag is flexible and able to reach out into solution away from the protein surface while maximizing the interaction between the tag and the matrix during purification