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Abbreviations ASCA anti- Saccharomyces cerevisiae antibody FGID functional gastrointestinal disorders GF germ-free IEB intestinal epithelial barrier IBD inflammatory bowel disease IBS irritable bowel syndrome IBS-C constipation-predominant irritable bowel syndrome IBS-D diarrhea-predominant irritable bowel syndrome IBS-M irritable bowel syndrome with mixed bowel habit LPS lipopolysaccharides SCFA short-chain fatty acids

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6,7 Paraffin sections of tumours were incubated with respective primary antibodies against human α-SMA (α-smooth muscle actin) (1:800), cytokeratin (1:75), PCNA (proliferating cell nuclear antigen 1:200), Ki-67 (1:333), DCLK1 (1:500), E-cadherin (1:200) and vimentin (1:3000), followed by incubation with relevant secondary antibodies and DAB visualisation